The enzyme contains thiamine diphosphate and two [4Fe-4S] clusters. Highly specific for 2-oxoglutarate. This enzyme is a member of the 2-oxoacid oxidoreductases, a family of enzymes that oxidatively decarboxylate different 2-oxoacids to form their CoA derivatives, and are differentiated based on their substrate specificity. For examples of other members of this family, see EC 18.104.22.168, pyruvate synthase and EC 22.214.171.124, 3-methyl-2-oxobutanoate dehydrogenase (ferredoxin).
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