EC |
2.3.1.253 |
Accepted name: |
phloroglucinol synthase |
Reaction: |
3 malonyl-CoA = phloroglucinol + 3 CO2 + 3 CoA |
|
For diagram of polyketides biosynthesis, click here |
Glossary: |
phloroglucinol = 1,3,5-trihydroxybenzene |
Other name(s): |
phlD (gene name) |
Systematic name: |
malonyl-CoA:malonyl-CoA malonyltransferase (decarboxylating, phloroglucinol-forming) |
Comments: |
The enzyme, characterized from the bacterium Pseudomonas protegens Pf-5, is a type III polyketide synthase. The mechanism involves the cyclization of an activated 3,5-dioxoheptanedioate intermediate. The enzyme exhibits broad substrate specificity, and can accept C4-C12 aliphatic acyl-CoAs and phenylacetyl-CoA as the starter molecules, forming 6-(polyoxoalkyl)-α-pyrones by sequential condensation with malonyl-CoA. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
References: |
1. |
Achkar, J., Xian, M., Zhao, H. and Frost, J.W. Biosynthesis of phloroglucinol. J. Am. Chem. Soc. 127 (2005) 5332–5333. [PMID: 15826166] |
2. |
Zha, W., Rubin-Pitel, S.B. and Zhao, H. Characterization of the substrate specificity of PhlD, a type III polyketide synthase from Pseudomonas fluorescens. J. Biol. Chem. 281 (2006) 32036–32047. [DOI] [PMID: 16931521] |
|
[EC 2.3.1.253 created 2016] |
|
|
|
|