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Your query returned 1 entry. Printable version
EC | 2.8.1.6 | ||||||||||||||||
Accepted name: | biotin synthase | ||||||||||||||||
Reaction: | dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5′-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin | ||||||||||||||||
Glossary: | biotin = 5[(3aS,4S,6aR)-2-oxohexahydro(4H-thieno[4,5-d]imidazol-4-yl)]pentanoate 4,5-secobiotin = 6-[(4R,5R)-2-oxo-5-(sulfanylmethyl)imidazolidin-4-yl]hexanoate = 9-mercaptodethiobiotin |
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Other name(s): | dethiobiotin:sulfur sulfurtransferase | ||||||||||||||||
Systematic name: | dethiobiotin:sulfur-(sulfur carrier) sulfurtransferase | ||||||||||||||||
Comments: | The enzyme binds a [4Fe-4S] and a [2Fe-2S] cluster. In every reaction cycle, the enzyme consumes two molecules of AdoMet. The first reaction produces 5′-deoxyadenosine and 4,5-secobiotin. Reaction with another equivalent of AdoMet results in abstraction of the C-6 methylene pro-S hydrogen atom from 4,5-secobiotin, and the resulting carbon radical is quenched via formation of an intramolecular C-S bond, thus closing the biotin tetrahydrothiophene ring. The sulfur donor is believed to be the [2Fe-2S] cluster, which is sacrificed in the process, so that in vitro the reaction is a single turnover. In vivo, the [2Fe-2S] cluster can be reassembled by the Isc or Suf iron-sulfur cluster assembly systems, to allow further catalysis. | ||||||||||||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 80146-93-6 | ||||||||||||||||
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